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KMID : 0380020180330030200
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Korean Journal of Biotechnology and Bioengineering
2018 Volume.33 No. 3 p.200 ~ p.208
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Antioxidant Activities of Lignin Extracted from Domestic Bamboo (Phyllostachys nigra var. Henonis) Stem
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Yang Seung-Hwa
Choi Moon-Hee
Shin Hyun-Jae
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Abstract
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Several fractions of lignin were extracted from the stem of domestic bamboo (Phyllostachys nigra var. henosis) through steam explosion and organic solvent fractionation. A maximum yield of 21% and 47% lignin were attained from steam explosion (SL) and non-soluble fraction (SL-F3), respectively. Molecular weights (and poly diversity index) and chemical structure consisting in the lignin were estimated by GPC, FT-IR, and NMR spectrometry. Major carbon-hydrogen bonding has been assigned by HSQC analysis. Among the four fractions, SL-F1 showed the highest polyphenol contents and antioxidant activities (DPPH, ABTS, hydroxyl radical and Fe2+ chelating activities). All four fractions showed no cytotoxicity up to the concentration of 100 μg/mL by MTT assay. The lignin fractions would be a valuable asset for many applications including cosmetic products.
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KEYWORD
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lignin, bamboo stem, steam explosion, solvent fractionation, antioxidant activity, cytotoxicity
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